Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid | MFCD00007532 | 250G
N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid, MFCD00007532, 250G
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 25G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 25G
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
![eMolecules Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502475470.JPG-250.jpg)
eMolecules Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5
Ambeed / (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride / 1g / 666597771 / A1152138 / / 1596117-29-1 / [null] / 237.690 / C10H12ClN5
PROVISION KINETICS INC TRIS- SALINE BUFFER
500 ml bottle of Tris-saline buffer, pH 8.4 designed for use with Anti-IIa and Anti-Xa heparin test kits (sold separately)
Quality Biological Inc DPBS without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Abcam Tris-HCl (pH 8.8), 100ML
A 1.5 M solution of Tris HCl in water (pH 8.8).
The product is subject to the following: Abcam Restricted Use Statement
Abcam PBS Tablets (1000 ml), 100TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 500G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 500G