Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris Glycine SDS Buffer, 10X, 4L Cubitainer
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Bioworld TAE Buffer (50X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 50X concentration and is filtered through a 0.22 µm membrane. It has a pH of 8.5 ± 0.1. A concentration of 1X contains 0.04M Tris, 0.02M acetate, and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
STEMCELL Technologies RoboSep™ Human CD11b Positive Selection and Depletion Kit, For processing: 1 x 10^9 cells
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Abcam 10X ANNEXIN V/ANXA5 BINDING BU ..
10X ANNEXIN V/ANXA5 BINDING BU ..
The product is subject to the following: Abcam Restricted Use Statement
Research Products International Corp TBE, 4 L Cube
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Teknova 10X TBS with Tween-20 Kit, 1000mL, Sterile. (1M Tris, 1.5M NaCl, 0.5% Tween)
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Tris/Glycine/SDS Buffer for Resolve Gel (1X), 20 PK(s)
1X Tris/Glycine/SDS Buffer for Resolve GelBuffer System: Pre-weighed Powder mix in pouches(packs)1 pouch(pack)= 500 mL
Research Products International Corp Triton Lysis Buffer Solution, 500 ML
1X buffer used to lyse cells under non-denaturing conditions.
1X Formulation:
NaCI: 150 mM
TritonX-100: 1%
EDTA: 5 mM
Alkali Scientific CellPro™ Tris Glycine Buffer 10X, 1L
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 100G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 100G