Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 25 G
ADA disodium is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers.
- Derived from adenosine deaminase
- Used in biochemical research
- Ideal for enzyme buffer preparation
Medchemexpress LLC Beta-hydroxyisovalerylshikonin | 7415-78-3 | 1 MG
Beta-hydroxyisovalerylshikonin is a natural product isolated from *Lithospermum erythrorhizon*. It acts as a potent inhibitor of protein tyrosine kinases (PTK) and is effective against a wide variety of tumor cell lines.
- Potent inhibitor of protein tyrosine kinases (PTK)
- Effective against various tumor cell lines
- Induces cell death in NCI-H522 and DMS114 cells
- For research use only
LICOR Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Dojindo Molecular Technologies Inc TAPS (25G), Good's buffer, CAS: 29915-38-6, pka=8.4, Active pH 7.7-9.1, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 50 MG
AR-13324 analog mesylate is an analog of AR-13324. AR-13324 is an ROCK and norepinephrine transporter (NET) inhibitor.
- Analog of AR-13324.
- Ar-13324 is a rock and norepinephrine transporter (net) inhibitor.
- Available in various sizes, including 50 mg.
Gold Biotechnology Inc MES Free Acid 1 kg
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659952.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 50 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a highly stable biochemical reagent used to prepare buffer solutions that maintain a consistent pH. This 50g quantity is ideal for laboratories requiring precise pH control in applications such as protein chemistry, enzyme kinetics, and electrophoresis. PIPES buffers are used in biological assays where maintaining a neutral pH (around 7.0) is critical for enzyme activity and the stability of proteins. This versatile buffer is commonly employed in cellular biology, microbiology, and various biochemical processes that need accurate and reliable pH regulation.
Dojindo Molecular Technologies Inc HEPES (100 G), Good's buffer, CAS: 7365-45-9, Purity: ≥99.0% (Titration), pKa=7.55, Active pH 6.8-8.2, made in Japan
Buffers perform an important task in biological experiments, often designed to keep pH within the 6-8 range where most biological reactions occur. Dr. Norman Good, the developer of HEPES, wanted to create buffers that prioritized this function, while also providing other benefits. Dr. Good produced a series of “Good buffers” – including HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) – that are nearly ubiquitous in their usage even now. The Good series of buffers share the following additional characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal-chelating capability. 5) High chemical stability. 6)Low absorption spectra in UV and visible regions.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
Medchemexpress LLC Heppso | 68399-78-0 | 99.9% | 5 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- It is a zwitterionic buffer.
- Its working pH range is 7.1-8.5.
- It displays relatively high ability to bind copper(II).
- It has a pKa of 7.84 at 2.0 mM buffer concentration.
- Available in solid form, white to off-white in color.
- Store powder at -20°C for 3 years or 4°C for 2 years.
- Store in solvent at -80°C for 2 years or -20°C for 1 year.
Medchemexpress LLC Iminodiacetic acid | 142-73-4 | 99.9% | 133.10 | 500 G
Iminodiacetic acid is a metal ion chelator that targets Cr6+, Cd2+, Ni2+, and Pb2+. It selectively and irreversibly binds metal ions through the coordination of carboxyl and imino groups, reducing the toxicity of metal ions and promoting their adsorption and separation. It is used for heavy metal ion removal, coordination complex stabilization, environmental pollution control (e.g., heavy metal adsorption in water), and coordination chemistry (e.g., metal ion detection and separation) research.
- Used for heavy metal ion removal
- Promotes adsorption and separation of metal ions
- Forms a stable 1:1:1 ternary complex with Pd2+
- Suitable for environmental pollution control research
- Suitable for coordination chemistry research
Molecular Dimensions 100 ML BOTTLE OF 1M MES
100 mL bottle of 1M MES (2-(N-Morpholino)ethanesulfonic acid monohydrate)
Medchemexpress LLC MES sodium salt (2-Morpholinoethanesulphonic acid sodium salt) | 71119-23-8 | 98.0% | 1 ML
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging from pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity around pH 6.15
- Poor affinity with metal ions
- Suitable for studies involving metal ions